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NGS methods are high-throughput DNA sequencing technologies permitting the sequencing of millions of DNA strands in parallel generating large volumes of sequence data in a relatively short period of time (Pettersson et al. 2009).
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In contrast, we permit the sequencing of exercise to be internally mentioned through agents' optimizing decisions.
The method permits the sequencing of the same 1% of all genomes, producing a multiple sequence alignment of 116,880 bases across 262 strains.
Here we demonstrated that this approach is also applicable to a pathogenic bacterium, A. phagocytophilum, and permits the sequencing of whole genomes without any need for strain isolation.
The high sequencing capacity of the Ion Proton permits the sequencing of all exons of 409 genes and ensures the detection of any mutation occurring in the entire coding region of the gene.
However, the MPSS technology permits the simultaneous sequencing of millions of signatures from a given library [ 5].
The presence of sequential fragment ions, a phenomenon of IRMPD, permit the comprehensive sequencing of the oligosaccharides studied in a single stage of activation.
Multiple complex mutations in the same DNA template can be introduced without intermediate subcloning, thereby permitting the generation of sequences with multiple mutations in a short period of time.
As discussed in [17], to accomplish this it is necessary to use an experimental model in which the transformation of a normal, immortalized cell by a single oncogene can be highly controlled, thus permitting the dissection of the sequence of events occuring after the initial alteration and the role of a particular gene or signal transduction pathway.
Efforts to differentiate these alternatives are only now becoming feasible with next generation sequencing (permitting the analysis of regulatory elements on a genome-wide scale) and studies of transcriptome responses to the environment.
It has also been the subject of an expressed sequence tag (EST) sequencing project, permitting the construction of cDNA microarrays [ 33].
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