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The multiplex PCR approach described here proved to be a species-specific, sensitive and fast tool for accurate diagnosis of clonorchiasis and/or opisthorchiasis, permitting the detection of their metacercariae in infected fishes or adult/eggs from patients in endemic areas.
The reaction allowed a detection limit of 1 fg DNA, corresponding to 0.01 parasite (data not shown), permitting the detection of 0.01 parasite per reaction.
This was performed with a Leica TCS NT confocal laser scanning microscope equipped with an ArKr laser, permitting the detection of signals from the fluorochromes with emission wavelengths at 488 nm and 568 nm.
Compared to microarrays, deep sequencing is independent of predefined probe sets, thereby permitting the detection of novel transcripts and alternative splicing [ 8].
Unlike cysteine, lysine residues are relatively abundant in proteins permitting the detection of every subunit of complex I and analysis of their conformation-dependent exposure.
To the best of my knowledge, there are no studies in marine systems that track phenotypic traits through time for >10 generations, permitting the detection of temporal changes.
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In addition, the greater sensitivity and specificity of this assay method has permitted the detection of episodes of fetal loss not detected by radioimmunoassay of urine specimens.
A sensitive quantitative polymerase chain reaction (QPCR) permits the detection of mRNA in small quantities that could not be detected by conventional methods used to detect proteins.
"Although the study may not have been large enough to permit the detection of any small effects," they wrote, "the data presented do not suggest that there were any.," In a telephone interview this week, Dr. Felson, a professor of medicine and a rheumatologist by training, praised the research but said it remained to be seen whether doctors and patients would abandon the procedure.
The characterisation of the oxidation kinetics has permitted the detection of interactions between the two polymers.
The use of Fe3O4@Ag nanoparticles (Fig. 14C) permits the detection of As contamination [86].
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