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This approach permitted the assembly of a nearly complete genome from metagenomic data derived from human stool.
Subcloning and partial sequencing of 5 BAC clones, plus the information obtained from the end sequences of the remaining five clones, permitted the assembly of the contig map shown in Figure 1.
Although mate pair sequencing permitted the assembly of the genome into a single circular scaffold, the final (~11) sequence gaps represent a very small fraction of the genome (a few hundred bases) and therefore are unlikely to impact our analyses.
Advancements in next-generation sequencing (NGS) technologies have permitted the assembly of large, genome-scale data sets that have shed light on the evolutionary history of many taxa (e.g., Parks et al., 2009; Moore et al., 2010; Xi et al., 2012; Eaton and Ree, 2013; Tennessen et al., 2013).
Individual phylogenies of these proteins confirmed that the components of these informational molecular machineries are little affected by LGT in the archaeal domain, and permitted the assembly of two large concatenated datasets of likely vertically-transmitted genes to reconstruct the phylogeny of the third domain of life [ 11].
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Their miniaturization permits the assembly of ultra-densely integrated circuits and faster processors.
Indeed, the likelihood that the species poorly represented will be covered by sufficient reads that will also permit the assembly of representative contigs is rather low.
Expressed protein ligation (EPL) and protein trans-splicing (PTS) are both intein-based chemical conjugation technologies that permit the assembly of a protein from smaller synthetic and/or recombinant unprotected polypeptide building blocks (Fig. 22).
On the other hand, the slower reaction of uranium-235 permits the assembly of super-critical masses, making it theoretically possible to produce weapons with high yields.
The PacBio technology now permits the assembly of actinomycete replicons into single contigs with >99%% accuracy.
Furthermore, the use of sequencing technology permits the assembly of the complete, or near-complete, genomes of pathogens from a sample.
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