We allowed cells to attach to monolayer substrates that presented SPRGDVC, KKKDLFEIFEIERC, or SPRGDVFIPRQPTNDLFEIFEIERC and then fixed the cultures to permit imaging of the cytoskeletal structures.
The hindlimbs were shaved, and the animals were placed in dorsal recumbency on a warming pad (37°C) with both hindlimbs abducted to permit imaging of the medial knee joint region.
In one study [ 116], Plectin1 targeted peptides (PTP) were conjugated to the surface of magnetofluorescent nanoparticles, and the results revealed that the targeted imaging agent PTP-NP permitted imaging of PDAC against the background of normal and ductal metaplasia of the pancreas.
The choice of vector permitted imaging of HNF6 (cloned into the MCS) by fluorescence microscopy.
We therefore used developing cortical slices that contained the entire callosal pathway through the sensorimotor cortex, which permitted imaging of intact callosal axons extending along their entire trajectory (Halloran and Kalil, 1994).
This imaging configuration provides an optical demagnification factor of 28/4 = 7-fold, which permits imaging of both the test and control cuvettes (6.6 × 6.6 mm in cross section) within the field of view of the phone's CMOS imager chip.
Both techniques have severe limitations; the former does not permit imaging of living cells, while the latter is a relatively artificial model of primary human neutrophils.
Furthermore, the zebrafish embryo is virtually transparent which permits imaging of internal organs, especially the heart, using standard light microscopy.
Some of the future developments with these techniques are expected to allow for 3D imaging with resolution as good as 5 nm3 voxel (coherent X-ray diffraction imaging), permitting imaging of whole frozen cells with the nanoparticles distributed at specific destinations in the cellular interior.
Although baselined for the monitoring of green fluorescent protein and chlorophyll fluorescence from Arabidopsis samples, the Flex Imager payload permits imaging of any biological sample contained within a standard 10 cm by 10 cm square Petri plate.
