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Internal initiation of replication and transcription from the second, internal, 3′ promoter directed the synthesis of subgenomic vRNA and mRNA and therefore permitted expression of the foreign gene product, e.g., the CAT enzyme.
In this mutant, the coding sequences for the two CsoS4 proteins were replaced by a kanamycin resistance cassette (Figure 2) that carries its own promoter and therefore permitted expression of the downstream shell proteins CsoS1C, CsoS1A and CsoS1B.
This potentiation occurred through insertion of the higher conducting GluR2-lacking AMPAR into both synaptic pathways, which subsequently permitted expression of metabotropic glutamate receptor (mGluR -dependent LTD through reinsertion of the lower conducting GluR2-containing AmGluR -dependentnapses [16].
A single exposure to cocaine potentiated both VTA local Glu-DA synapses and PPN Glu-DA synapses through insertion of the higher conducting GluR2-lacking AMPAR into both synaptic pathways, which subsequently permitted expression of mGluR-dependent LTD through reinsertion of the lower conducting GluR2-containing AMPAR at these synapses [16].
The resulting recombinant GRA5-pRSET B construct permitted expression of an N-terminally polyhistidine- (His-) tagged rGRA5 (amino acid residues 26 120), lacking its putative N-terminal signal sequence.
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These utilize a host cell line to express the desired collagen gene of interest, permitting expression of mutated genes and also of completely novel protein sequences.
This versatile system permits expression of incorporated antigens in either surface-bound or secreted forms by the ZCR533 vector, for delivery to the mucosal inductive sites.
Adjacent to the A-MLV envelope is an IRES that permits expression of GFP, schematically depicted in Figure S1B.
The introduction of a loxP-flanked neomycin resistance (neor) cassette and polyadenylation signal into the intron upstream of the site B mutation prevents expression of the mutant RIα allele; however, in the presence of Cre recombinase, excision of the floxed neor cassette permits expression of mutant RIα.
Recombination requires transfection with two plasmids: pINT that expresses the mycobacteriophage Bxb1 integrase and a neomycin selectable marker conferring resistance to G418, and pLN-ENR-GFP that permits expression of a gene of interest fused to gfp and that contains the mycobacterial attP recombination sequence as well as a blasticidin resistance cassette.
Short days permit expression of the inhibitory neurohormones and, ultimately, result in non-developing ovaries (diapause).
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