A reporter for cAMP has been incorporated into the genome of mice, under a tightly regulated promoter that permits expression in any cell type of interest, and only upon activation with doxycycline.
The full-length coding region of the Human PACT gene was cloned into the pUASP vector under the transcriptional control of the UASp element, a modified form of UAS that permits expression in both somatic and germline cells.
The importance of open emotional expression within the family is underscored by findings that strong emotions that are not permitted expression can "go underground" and emerge in a destructive fashion through emotional, behavioral, and even somatic symptoms (Greene et al. 2003).
The progeny then express the UAS transgene of interest in the pattern determined by the GAL4 'driver' stock, permitting expression of genes in specific cell types at specific stages of development.
HGT of fused proteins has been reported previously [ 37, 38], and the putative NrdDG fusion raises the possibility that subunit fusion facilitates successful fixation of transferred prokaryotic operons in eukaryotes by permitting expression of distal genes in operons for which eukaryotic promoter sequences and translation initiation sites will be unavailable.
This versatile system permits expression of incorporated antigens in either surface-bound or secreted forms by the ZCR533 vector, for delivery to the mucosal inductive sites.
Accordingly, selection for atherosclerosis resistance may not have altered the expression of ABCG5 but rather the expression of some of these receptor genes [ 26, 28, 29] thus permitting expression of ABCG5 to remain in an up-regulated state in the RES individuals.
Adjacent to the A-MLV envelope is an IRES that permits expression of GFP, schematically depicted in Figure S1B.
Short days permit expression of the inhibitory neurohormones and, ultimately, result in non-developing ovaries (diapause).
As a first step we cloned the mRFP1 gene into a vector permitting widespread expression in a variety of cells types in culture and in vivo in mice.
