Observation of semithin sections of the seabream gut tissue during the different steps of the isolation protocol permitted visualisation of the different degrees of digestion of the tissue as well as the progressive release of immune cells.
On day 20 after tumour injection tumour-bearing animals were killed with carbon dioxide, and dissected with a midline skin incision extending from the caudal abdomen to the lower jaw, the skin was reflected and an incision into the abdominal cavity just cranial to the external genitalia permitted visualisation of the viscera.
Live cell imaging has been widely used to investigate neuronal dysfunction in cultured cells in vitro, which, together with fluorescent multiple labelling, permits visualisation of different cell activities and distinct molecular localisation patterns.
The dorsal skin of each mouse was harvested to permit visualisation of intradermal dye leakage.
Moderate background uptake is generally present in the liver, muscles, small intestine and bone marrow, but this background uptake is (sufficiently) low to permit visualisation of tumour lesions.
No staining agents were necessary to achieve high-resolution, high-contrast images, which permitted visualisations of both calcified and soft tissue.
It avoids the need to puncture the cisterna magna and permits visualisation and timing of CSF distribution and drainage, quantification of CSF flow elsewhere within the ventricular system and measurement of total brain and intracranial CSF volumes.
Careful incision placement is required together with skin retraction to allow visualisation of the tendon ends and to permit the reconstruction.
Bright field illumination permits the visualisation of the total number of sperm, whereas epifluorescence illumination permits the visualisation of the fluorescent sperm only.
