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In this scenario, multiplex PCR has a significant advantage, as it permits simultaneous detection of several viruses in a single reaction mixture, facilitating cost-effective diagnosis [ 11].
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It has already been demonstrated that automated CLIA, FEIA or Luminex analytic instrumentations permit the simultaneous detection of various single autoantibodies, achieving quantitative results in under an hour.
All the species were amplified homogeneously, and a homogenous band ladder was observed, indicating that the assay permitted the simultaneous detection of all the species in a single-tube reaction.
Experimental results revealed that the sandwich-type immunosensor can permit the simultaneous detection of CEA and AFP with linear range of 0.6 80 ng mL−1 for two analytes, the detection limit was 0.12 ng mL−1 for CEA and 0.08 ng mL−1 for AFP (at a signal to noise ratio of 3), respectively.
However, we have uniquely developed duplex and triplex HRM assays to permit the simultaneous detection of multiple targets per reaction.
It also permits for the simultaneous detection of Alphaproteobacteria, Gammaproteobacteria, Verrucomicrobia and NC10 phyla with broader sequence variation.
It is a nondestructive, very sensitive, and multielemental technique which permits rapid and simultaneous detection of elements between Na and U [ 10, 11].
This technique permits simultaneous determinations of adsorbed amounts and interaction energies.
Although selectable traits are limited, currently each trait only permits simultaneous introduction of a single DNA fragment.
The sensor was also equipped with a thermocouple and tubing that permits simultaneous measurement of temperature and gas sampling.
This permits simultaneous identification of the cell type and the activation state in addition to other phenotypic markers.
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