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Although baselined for the monitoring of green fluorescent protein and chlorophyll fluorescence from Arabidopsis samples, the Flex Imager payload permits imaging of any biological sample contained within a standard 10 cm by 10 cm square Petri plate.
To examine those questions we used recombinant adenovirus to express a synaptophysin-GFP (Green Fluorescent Protein) fusion protein, which permits imaging of individual puncta over time in living neurons.
Furthermore, the zebrafish embryo is virtually transparent which permits imaging of internal organs, especially the heart, using standard light microscopy.
This imaging configuration provides an optical demagnification factor of 28/4 = 7-fold, which permits imaging of both the test and control cuvettes (6.6 × 6.6 mm in cross section) within the field of view of the phone's CMOS imager chip.
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The results suggest that a detector array of 0.5 cm×0.5 cm×5 cm pixels of bismuth germanate may provide sufficient efficiency and spatial resolution to permit imaging of an object with uncertainties in dimension of several millimeters.
Some of the future developments with these techniques are expected to allow for 3D imaging with resolution as good as 5 nm3 voxel (coherent X-ray diffraction imaging), permitting imaging of whole frozen cells with the nanoparticles distributed at specific destinations in the cellular interior.
The optical efficiency of the system permitted imaging of the human oral mucosa in real time.
The choice of vector permitted imaging of HNF6 (cloned into the MCS) by fluorescence microscopy.
Both techniques have severe limitations; the former does not permit imaging of living cells, while the latter is a relatively artificial model of primary human neutrophils.
We allowed cells to attach to monolayer substrates that presented SPRGDVC, KKKDLFEIFEIERC, or SPRGDVFIPRQPTNDLFEIFEIERC and then fixed the cultures to permit imaging of the cytoskeletal structures.
The hindlimbs were shaved, and the animals were placed in dorsal recumbency on a warming pad (37°C) with both hindlimbs abducted to permit imaging of the medial knee joint region.
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