For low cell density SP analyses, trypsinized cells (prepared as described above) were plated at 500 cells/cm2 in 2-well LAB-TEK® chamber slides in Zn-free medium (Nunc, Inc., Naperville, IL) to permit sister-sister designation based on close relative proximity.
Out with a girl in 1973, Alexander Umnitzer reflects that her flashy outfit was only possible because of the recent Basic Treaty, in which the Federal and Democratic republics acknowledged each other's sovereignty – and permitted sisters to send each other leather coats and "strikingly short" acrylic skirts across the inner German border.
If this explanation is the case, abolition of sister-chromatid cohesion should permit the sister centromere with an active kinetochore to be drawn to a spindle pole, but should leave the other sister centromere lacking an active kinetochore within the middle of the nucleus.
Their bosses argued over whether to issue a permit to the Sisters of Perpetual Indulgence, an irreverent theater group whose members dress as nuns, to hold a street party on Easter Sunday.
Separase, which is repressed by securin, becomes activated and cleaves the components of cohesin to permit the segregation of sister chromatids (Schvartzman et al., 2010).
His mother permitted him & his sister to take along their Belgian hares, Buck & Mollie.
It is surprising that Morisot's bourgeois parents permitted her and her sister, Edma, to study art.
Societal norms regarding how friendly a brother and sister are permitted to become are also largely ignored.
Had Leo read it, he would never have permitted Elena or her sister Zoya to travel to the United States on a school choir tour organised by his wife.
So it took a lot of work to opt out the child with special needs, but her younger sister was permitted to refuse to sit for the test on her own.
With the usual irony she asks how some heretical lodges could be permitted to accept "sisters" after a battle lasting three centuries.
