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Transformation efficiencies are sufficiently high to permit screens using expression libraries.
The Zebrafish lgi1 mutants will permit screens for small molecules that ameliorate their abnormal phenotypes, whereas the Lgi1 mutant rats will be useful for preclinical testing of candidate antiepileptic drugs.
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Because of their large arm sizes, however, BACVecs do not permit screening by conventional assays, such as long-range PCR or Southern blotting, that link the inserted targeting vector to the targeted locus.
Microsatellites are commonly used in genetic studies, but new methods, including restriction site associated DNA sequencing, now permit screening thousands of loci at comparable costs.
Finally, the hypomorphic nature of the tefu ZIII-5190 allele should permit screening of enhancer/suppressor mutations, facilitating the genetic identification of new members of the ATM pathway.
129 Another advance is whole genome or exome sequencing, which would, in theory, permit screening of the entire genome or protein-coding region.
The rapid progress in advanced sequencing technologies will permit screening approaches to identify causative uORF mutations in primary material derived from patients.
These technology platforms permit screening of carbohydrate structures in combinatorial systems and offer the possibility to explore subtle α-glucan structural alterations in situ in living organisms including plant mutants and medical patients.
The differential expression and gene families databases permit screening and extraction of the entire dataset down to the single gene level, aided by application of a look-up and plotting tool.
The vectors developed permit screening for the desired insertion through PCR or direct selection of intron insertions using a selection scheme that activates a kanamycin resistance marker upon successful intron insertion.
10T1/2 cells stably transfected with a luciferase reporter gene driven by multimerized Gli binding sites responded strongly to addition of exogenous Shh, permitting screens both for inhibitors (in the presence of Shh) and activators (in the absence of Shh).
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