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For the case of Fe3O4 nanoparticles that are 10 nm in diameter, the SiNT sidewall pore dimensions are insufficient to permit loading by diffusion through this orifice and thus the SiNT film must be removed from the substrate prior to loading of this sample.
DCs were cultured with Mel888 cells for 48 h, either in the presence or absence of LAKs, at 1 : 3 : 10 ratio, respectively, to permit loading of antigen on to the DCs, as previously described (Errington et al, 2006).
Ideally, the homogenates would be diluted to a concentration of at least 2 mg/mL which would permit loading between 10 μg and 80 μg per lane of a 1 mm thick mini polyacrylamide SDS-gel.
In this protocol, cells were first perfused in HC buffer to permit loading of internal stores (HeLa cell responses were highly variable and inclusion of a loading step with HC buffer was found to maximize signal-to-noise in many of our assays).
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He suggested consulting a physical therapist or exercise physiologist to help design "an exercise program that permits loading joints appropriately".
The second surprising aspect of this observation is that cardiolipins are confined almost entirely to the inner membrane of the mitochondria where they are synthesized from diacylated precursor phospholipids [33], and therefore their presence in the CD1d ligand pool suggests that they are transported from mitochondria to a location that permits loading into CD1d molecules, such as the ER.
A road sign tells drivers not to exceed the permitted load limit.
Articular cartilage is a mechanically and structurally complex, lubricious tissue that permits load-bearing and frictionless movement of our joints upon articulation.
The curves would also need to be fixed at Geita Bridge, but this was not done since the trams exceed the bridge's permitted load.
Sequential activation of the valves, worm and fluid inlets permit sequential loading of individual worms for imaging.
One possibility is that Cdc6 stabilizes binding of ORC to weaker sites during G1 to permit MCM loading [ 45, 46].
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