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We confirmed that Ae2-GFP was present in the plasma membrane of GV oocytes that were kept arrested for 20 hours (with dbcAMP) to permit expression (Figure 4A).
Short days permit expression of the inhibitory neurohormones and, ultimately, result in non-developing ovaries (diapause).
There are now three independent binary transcription systems — GAL4 UAS [34], LexA lexAop [35] and QF QUAS [36] — that permit expression of a variety of label and effector transgenes (see below).
To generate reliable and versatile constructs to transduce primary CD4+ T cells that permit expression of FoxP3 and Bcl-xL genes, we used the T2A peptide to generate a retroviral vector with efficient translation of two cistrons (for example, FoxP3 and Bcl-xL).
During interphase, the genome is packaged to permit expression of active genes and facilitate the silencing of inactive genes, all while maintaining plasticity to allow for variation in gene expression in response to the environment (reviewed in Branco and Pombo 2007; Heard and Bickmore 2007).
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These utilize a host cell line to express the desired collagen gene of interest, permitting expression of mutated genes and also of completely novel protein sequences.
Twelve months of reflection on American policy in the Middle East and scrutiny into how the United States did or did not protect itself have yielded recriminations and resentments -- many of them familiar, some more barbed than in the past, all permitted expression by the passing of a year's time.
Customarily located in a tower, it is played from a clavier, or keyboard, containing wooden levers and pedals wired to clappers or, less commonly, from an ivory keyboard with electric action operating the clappers; but only the first method permits expression through variation of touch.
This versatile system permits expression of incorporated antigens in either surface-bound or secreted forms by the ZCR533 vector, for delivery to the mucosal inductive sites.
Internal initiation of replication and transcription from the second, internal, 3′ promoter directed the synthesis of subgenomic vRNA and mRNA and therefore permitted expression of the foreign gene product, e.g., the CAT enzyme.
Adjacent to the A-MLV envelope is an IRES that permits expression of GFP, schematically depicted in Figure S1B.
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