Monovalent binding reduces avidity and potency, thus expanding the range of mutations permitting antibody evasion.
The efficacy of antibody-MCC-DM1 conjugates depends not only on internalization, but also on effective delivery to lysosomes, permitting antibody degradation and DM1 metabolite release (Erickson et al, 2006).
We hypothesized that Fc-Fc interaction between IgG3 molecules confers a greater effective valence, and thereby greater functional affinity, permitting antibodies with relatively modest intrinsic (i.e., per site) affinities to bind effectively to highly multivalent antigens [ 21].
Nevertheless, given the indispensable role for BLyS in B-cell development [ 9, 10], a certain threshold level of BLyS is required to permit any antibody responses (including autoantibody responses).
We conclude that VP1 based antigens combined with adjuvants may permit successful antibody-mediated vaccine design and development.
Substitution of these large non-polar side chains with methyl groups either increases solvent accessibility around K86 and/or permits greater antibody affinity in the second luminal loop of FIT2, as indicated in limited trypsin digestion experiments, correlating with increased FIT2 activity (Fig. 7).
The reactivity of the MAb C595 with synthetic peptides containing this motif permits efficient antibody purification using peptide-epitope affinity chromatography, which, unlike other methodologies, enables exclusion recovery of functionally active antibody.
Sensitive solid phase immunoassays for HLA-specific antibodies permit analyses that guide both desensitization and donor exchanges through antibody monitoring, virtual crossmatches, and detection of cryptic sensitization.
Finally, grafting of epidermis reconstituted in vitro on dermal substrates to SCID mice permitted sustained monoclonal antibody delivery into the bloodstream to be achieved.
