Sentence examples for permit amplification from inspiring English sources

Thus the locus-specific primers were designed downstream of this suspected intron position to permit amplification of these gene sequences from gDNA.

Taken together, for reliable Helicobacter species detection and distinction, it would be advisable, although seldom done so far, to use multiple PCR primer sets at somewhat reduced annealing temperatures to permit amplification of imperfectly matching sequences.

During DNA amplification the short (30 s) polymerase extension time did not permit amplification of wild-type PCR products, allowing only amplification of the shorter and deleted mtDNA fragments.

Although primers exist that will permit amplification of most enteroviruses (22, 23 ), few multiplex systems can identify all genotypes and serotypes within, or between, the different families of viruses that infect humans (22, 24, 25 ).

Amplification of DPP from European hedgehog, eastern hedgehog, northern shrew, ornate shrew, sperm whale, as well as three animals that are toothless in adulthood (pangolin, fin whale, and echidna) was unsuccessful suggesting that one or both primer-annealing sites may occasionally become sufficiently unlike any of our current primer-pairs to permit amplification in the PCR reactions.

This is important as the genetic basis for amplification remains obscure, although presumably certain deficits in DNA repair, replication, or cell cycle checkpoints are required to permit amplifications to occur.

A single set of primers was designed that permitted amplification of all three transcripts and the internal standard simultaneously; differentiation of the individual transcripts was achieved by restriction enzyme digestion.

This permits amplification of viral genomes from highly divergent viruses for which robust consensus primers focus on conserved regions are difficult to design.

The resulting cDNA is circularised resulting in PCR primer sites either side of the fragment permitting amplification [ 2].

This technique relies on hybridisation of probes on either side of a target sequence and subsequent ligation that permits amplification.

Problems in eliminating PCR inhibitors from stool specimens have been extensively reported and, for many situations, dilution of inhibited samples proves necessary to provide a rapid and straightforward way of permitting amplification [ 31].