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We were able to identify one such mouse line, MTH-Cg2-19 [23], weich we label as "permissive line" because tTA-dependent Ptetbi activation in these mice occur earlier compared to "tight" lines (compared Fig. 5A-upper panel with Fig. 5B-upper and lower panels, also see Fig. 5C).
At present, no permissive line for PhcyNPV is available.
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To investigate whether such a complex existed in MHV-A59 infected cells, we used a MHV-A59 permissive cell line 17Cl-1 and an engineered HEK293T cell line stably expressing MHV-A59 receptor HEK293T-mCEACAM-1 HEK293T-mCEACAM-1 HEK293T-mCEACAM-1d above.
Here again, the amount of hexon protein produced 48 hours after infection in the permissive cell line T47D outperformed that produced in nonpermissive cell lines MDA-MB-231.
In fact, in Huh-7 cell lines, the only permissive cell lines for HCV infection, genotype 2a did show a proliferation rate higher than that of other HCV genotypes 21, 22 in agreement with our observations.
The main purpose to generate Jurkat-KI-R5 cells is to create a permissive cell line for HIV infection.
However, NoV vaccine development has faced many difficulties, including genetic/antigenic diversity, limited knowledge on NoV immunology and viral cycle, lack of a permissive cell line for cultivation and lack of a widely available and successful animal model.
For use in animal studies, virus stocks underwent purification via centrifugation through a 36% sucrose cushion prior to resuspension in PBS and titration via plaque assay on a commonly permissive cell line.
Viral particles are obtained by transfection of BAC-cloned viral DNA into a permissive cell line.
Most ORFs were expressed at 'sooner and higher' levels in a more permissive cell line.
Alternatively, it may mean that these changes could only be fixed by drift in the permissive cell line environment.
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