Iodide permeates VRAC efficiently and hence VRAC opening increases YFP quenching rates.
Functional characterization reveals that, although each paralog permeates water efficiently, the permeability is respectively shifted to the neutral, alkaline, or acidic pH in Aqp0a1, -0a2, and -0b1, whereas that of Aqp0b2 is not regulated by external pH.
The active molecule 7h could not only rapidly kill C. albicans but also efficiently permeate membrane of C. albicans.
These observations provide evidence that scImpβ could not only bind to but also efficiently permeate the FG repeat film.
They find that NTRs can efficiently permeate the films, but do not affect their global morphology, which suggests that the FG repeat domains form a dense meshwork of entangled or transiently crosslinked polymers.
Nevertheless, our results indicate that oocytes expressing DrAqp3b and exposed to isotonic or hypertonic ethylene glycol solutions permeate this cryoprotectant more efficiently than those expressing HsAQP3.
We observed that scImpβ can efficiently enter, permeate and leave FG repeat films, and quantified the impact of cargo and Gsp1pGTP on binding.
To confirm that DrAqp3b-expressing oocytes permeated ethylene glycol more efficiently than those expressing HsAQP3, oocytes injected with 20 ng DrAqp3b or HsAQP3 cRNA were exposed to radiolabeled ethylene glycol.
Altogether, these results suggested that DrAqp3b was more permeable to ethylene glycol than to propylene glycol or glycerol, and that DrAqp3b oocytes permeated ethylene glycol more efficiently than those expressing HsAQP3.
In addition, dose-response experiments and radiolabeled ethylene glycol uptake assays suggested that oocytes expressing DrAqp3b were permeated by this cryoprotectant more efficiently than those expressing AQP3.
