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DSP is a homobifunctional cell permeable cross-linker.
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Antp-LP4-treated MEC-1 cells were subjected to chemical cross-linking with the membrane-permeable cross-linker, ethylene glycol-bis(succinimidyl succinate) (EGS).
24 hr post transfection proteins were cross linked by incubation with the cell permeable cross linker DSP Piercee, Rockford, IL USA) for 30 min at room temperature.
To address this issue, Jurkat cells were exposed to varying concentrations of NPM or 500 nM of staurosporine (as a positive control for inducing Bak oligomerization) for 3 h and then treated with membrane-permeable protein cross-linker BMH.
Network formation was analyzed for varying cross-linker reaction rates, cross-linker composition and number fraction, cross-linker functionality and time dependent initiator release.
The DAK cross-linker was synthesized as previously reported [43].
This effect is stronger for microgels containing less cross-linker.
Further, higher concentration of rigid cross-linker or lower concentration of flexible cross-linker enhanced the thermal stability.
Copolymer containing rigid cross-linker (divinylbenzene) had better thermal stability over copolymer with flexible cross-linker (ethylene dimethacrylate).
The cross-linker effectively reduced the on-set curing temperature.
PEI acts as a cross-linker between the fibrils, and thus, the length of the cross-linker will influence the properties of the formed material.
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