Sentence examples for permeable calcium from inspiring English sources

Cells were then loaded with the cell permeable calcium indicator Fluo-4 AM (F-14201, Invitrogen).

To examine whether the ErbB1- dependent inhibition of neurite outgrowth by poly I C is similarly dependent on intracellular calcium signals, we performed outgrowth assays in the presence of the cell permeable calcium buffer BAPTA-AM.

Cultured TG neurons were loaded with 1 μM of the cell permeable calcium sensitive dye, Fura 2 AM (Molecular Probes) for 30 min and washed with HBSS before use.

As depicted in Figure 6a, the cell permeable calcium-chelator Bapta-AM completely abrogated cyclin D1 expression induced by cimes.

In some experiments pre-incubations with the calpain inhibitor MDL28170 (Sigma, Taufkirchen, Germany) or the cell permeable calcium-chelator Bapta-AM (Invitrogen, Karlsruhe, Germany) were performed as indicated.

This hypothesis was subsequently shown to be correct as the membrane-permeable calcium chelator BAPTA-AM reduced 3O-C12-mediated IL-6 production in both cell lines.

To demonstrate the technique, we exposed live fibroblast cells to ionomycin, a membrane-permeable calcium ionophore, while assaying cytosolic calcium concentration.

To investigate if the 3O-C12 inducelevationsons and sustained increases in [Ca2+]i in IB3 were responsible for differential induction of proinflammatory cytokines in these two cell types, cells were stimulated with increasing doses of 3O-C12, with or without a 1-hr pretreatment with membrane-permeable calcium chelator BAPTA-AM.

Pretreatment of HL-1 cells with either EGTA (1 mM) to chelate extracellular calcium or with the cell-permeable calcium chelator, BAPTA-AM (1 nM), each sufficient to inhibit contractile activity, had no effect on cell death analyzed by either trypan blue or TUNEL staining (data not shown), suggesting that the induction of cell death did not depend on significant levels of intracellular calcium.

Therefore, neurons were treated with the cell-permeable calcium chelator BAPTA-AM at a concentration that has been shown not to affect neuronal viability [ 21].

We further observed that reducing intracellular Ca2+ by cell-permeable calcium chelator BAPTA-AM did not affect the peak time of expression (Fig. 3 A, B).