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Finally, manipulating transgene subcellular localization, we found that provided we avoid transgene expression in antigen presenting cells, the poorly accessible cytosolic form of ovalbumin transgene lacking strong MHC II epitope, evades CD8+ T-cell priming and remains permanently expressed in muscle with no immune cell infiltration.
To verify its sub-cellular localization, CPR5 was fused (C-terminal) with EGFP and permanently expressed in cpr5-1 munder under the control of the CaMV 35S promoter.
Permanently expressed 'housekeepers' can be split into different subgroups related, e.g., to protein metabolism, mitochondrial or transcriptional activity due to tissue-specific modulation of their expression levels.
Of note, integrin α-4 was not permanently expressed under basic culture conditions in vitro, but intermittently undetectable in the majority of positive preparations including the cell line.
All other variants were less subject to variation, and appeared to be permanently expressed on a lower level over the Stylonychia life cycle.
NO is produced by various NO synthase isoforms (NOS), such as neuronal NOS (nNOS) and endothelial NOS (eNOS), which are permanently expressed in skeletal muscle.
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To prepare a cell line permanently expressing TRPV1ε, HaCaT cells were transfected with the pMTH-TRPV1ε plasmid using the Exgen 500 transfection reagent (Fermentas, Burlington, Canada).
It is already known that some Antarctic species permanently express the inducible form of HSP70 as well as other HSP70 family members [63] [69].
Functional assays were carried out in TRPV1-NIH3T3 cells permanently expressing the receptor ectopically, and in primary cultures from embryonic rat dorsal root ganglia (DRG), enriched in TRPV1+ nociceptor/pain neurons.
Secondary PTMs of exogenous proteins is unavoidable from either transiently or permanently expressing cells.
We established Flag-CHOP-LiSa-2 cells which permanently express Flag-CHOP (Fig. 2A).
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