Sentence examples for permanent mount from inspiring English sources
Slides were washed with distilled water, dried and cover-slipped using permanent mount (Novocastra).
The slides were subsequently placed in decreasing concentration of alcohol and finally mounted in DPX permanent mount.
Then, cells were rinsed in distilled water, dehydrated through alcohol and xylene and mounted on a coverslip using a permanent mount medium for analysis by a microspectrophotometer (Leitz Dmrbe, Leica).
Vidikron's DP100 requires permanent mounting.
Slides were air-dried, dehydrated through graded ethanol solutions (70%, 95 % and 100), incubated in xylene and coverslipped using Eukit (Proscitech, Queensland, Australia) permanent mounting media.
Samples were dehydrated through a series of alcohols and xylene and mounted with the permanent mounting medium Permount (Fisher).
Slides were counterstained with hematoxylin QS Vector Laboratoriess) and were either mounted with low-viscosity aqueous mounting medium (Scytek Laboratories, Logan, UT) or dehydrated and mounted with VectaMount Permanent Mounting Medium Vector Laboratoriess).
Permanent mounts were heated to 65°C for 1 min. Stain solution was removed with a transfer pipette, and slides were rinsed with sterile, deionized water until no dissolved stain was visible in the rinse solution.
Sections within the circled area were stained by covering with a drop of lactophenol cotton blue (BD Diagnostic Systems, USA, Product number 261188) for wet mounts, or a drop of either trypan blue stain (0.05% trypan blue, 50% glycerol, and 0.05% hydrochloric acid in water)., or 0.5% toluidine blue in water for permanent mounts.
The following day, slides were washed, incubated in Mouse Polymer-HRP (BIOCARE MM HRP- KIT ref: MM510G) for 20 minutes, and treated with the chromogen diaminobenzidine tetrahydrochloride (DAB), counterstained with hematoxylin, dehydrated through grades of alcohols and xylenes and mounted with permanent mounting media.
Coverslip slides with permanent mounting media.
