As chromogens we used DAB+ and Liquid Permanent Red Kit (both from Dako).
The Envision G/2 System/AP (permanent red) kit (DAKO) was used to visualize VSMC.
I just wore black, but Danielle Willis was a Satanic blood fetishist who had her own 19th-century phlebotomy kit, permanent fangs dentally bonded to her eyeteeth and serious drug problems.
Sections were then stained with DAB for visualizing cytokeratin colored brown and with fast red (Permanent AP red kit, Zytomed, Berlin, Germany) for visualizing CAV1 colored purple.
Antigen retrieval for Twist was performed by heating the slides in sterile water at 92° for 10 min and cooling for 20 min, followed by exposure to the Twist antibody at 4°C overnight and thereafter conjugation with rabbit/mouse polymer (Super-picture HRP, Invitrogen, Frederick, MD, USA) for 30 min at RT. Twist was finally visualized colored green (Permanent HRP green kit, Zytomed, Berlin, Germany).
Tough as a vault, these handsome new cases are now a permanent part of my kit".
Permanent AEC (permanent AEC Kit, Zytomed Systems) was used as substrate for HRP-conjugated polymer.
Now equipped with a permanent limp and a prosthesis, Kit wants to thank Josie Schaeffer, the widow of a sergeant who has been mentioned several times earlier in the book.
As I look back on all the products I tried, loved, hated and dribbled over in 2014, it occurs to me that several of them not only made the grade, but have also taken up permanent residence in my everyday kit.
