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Induction of Cre recombination by tamoxifen results in permanent expression of YFP in Bmi1-expressing cells and their progeny.
P-cadherin is transiently expressed in various tissues during development [ 17, 18] and its permanent expression is limited to adult epithelial tissues, at cell-cell boundaries [ 19].
After binding with tamoxifen, Cre recombinase translocates into the nucleus to mediate Cre loxP recombination, thus eliciting permanent expression of the EYFP reporter gene in the Plp promoter-driven EYFP-expressing progenitors and their progenies.
The short periods of time during which shRNAs are sequentially expressed will reduce any negative effects that could be induced by their permanent expression within the retina.
He wore the permanent expression of a man expecting incoming fire.
He wears a mustache that curls down the sides of his upper lip in a permanent expression of melancholy.
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The location is suitable for a semi-permanent expression of article 10 and article 11 rights.
Cells expressing the cardiomyocyte-specific alpha-myosin can be genetically labeled by an inducible recombination technique leading to permanent GFP expression of only cells that expressed alpha-myosin at time of induction [ 23].
With his permanent hangdog expression, he's hardly an inspirational figure himself.
Unfortunately, it's all rather grey and slow-paced with Gabriel Byrne wearing a permanent facial expression that suggests he's sitting through a recitation of the periodic table.
Everyone, including Mr. Giuliani, would like to see a ground zero memorial as powerful as Ms. Lin's, but it's hard to imagine she or anyone could have created so permanent an expression of that war in its immediate aftermath.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com