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Democracy demands permanent agitation.
Incubation was performed overnight at 4°C under permanent agitation.
MagnaBind magnetic beads (Pierce, Thermo Fisher Scientific, Bonn, Germany), pre-coated with anti-rabbit IgG antibody, were incubated with anti-irisin antibody (Adipogen) for 60 min under permanent agitation (Dynabeads MX Mixer, Life Technologies, Darmstadt, Germany).
The Cy5 dye was added to the antibody while vortexing and further on incubated under permanent agitation for 1 h at RT (light protected).
Undifferentiated cells of UTF-1, UTF-2 and UTF-3 were cultured in suspension during permanent agitation to allow formation of embryoid bodies.
After the centrifugation, the supernatants were incubated with TALON resin for two hours at 4°C with the permanent agitation.
Similar(53)
The pro-Israel lobby lives in permanent nervous agitation.
His followers, drawn from the 80percentt of Venezuelans who live below the poverty line, have been kept in a permanent state of agitation.
Along the way, Mr. Chavez kept his followers -- the vendors and factory workers, the maids and truckers who closely identified with him -- in a permanent state of agitation.
Her agitation made me agitated.
While it's not permanent like diabetes or smoking, it can cause agitation worth addressing.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com