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The pERK time response curve should be smooth and not oscillate like an under-damped second order control system.
Based on the experimental data, the following constraints were incorporated into the objective function 4. The pERK time response curve should reach a peak between 4-10 min after ligand stimulation if t peak > 10 min : p 4 = t peak 7 ; if t peak < 4 min : p 4 = 7 t peak where t peak) is the time point of the peak of the pERK time response in minutes. 5.
If there were more than 3 peaks, the following penalty was defined: p 5 = ∏ i = 1 m 4 θ i - 1 where m is equal to the number of FGF concentrations and ϑ(i) is the number of peaks in pERK time response to FGF2 concentration i. 6. pERK dose-response curve should be biphasic at certain time-points as observed in experiments.
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The expression levels of nuclear Six1 and levels of ERK phosphorylation (both nuclear and cytoplasmic pERK, intensity times percent of area) were significantly correlated (P < 0.05), These findings demonstrate that Six1 correlates with pERK in human breast cancer, and suggest that activation of ERK by Six1 may lead to expansion of TICs and to increased tumor aggressiveness.
This artiCle originally appeared on VICE US.
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