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We initially expressed and purified full-length human Ire1 and Perk luminal domains along with full-length BiP encompassing both the ATPase domain and the substrate binding domains.
To validate the observed changes in pERK activity, a 20 kb genomic rescue construct encompassing the 6 kb RSK transcription unit (in the following, termed P[RSK]) was introduced into a RSK Δ58/1 background.
All encompassing.
That encompasses about everything.
But it encompasses all.
One cannot encompass everything.
"She encompasses it all".
It encompasses many fields.
That encompasses about 50 banks.
-- encompasses everything.
which encompassed my life.
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