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Similarly, NK cells that were co-cultured with HEK293 expressing GFP-H-RasG12V displayed higher levels of pERK compared with NK cells co-cultured with HEK293 expressing the inactive GFP-tH (Fig. 5f).
In addition, FACS analysis (Fig. 5e) indicated that NK cells that acquired GFP-H-RasG12V exhibit a higher increase in the intracellular levels of pERK compared with NK cells that acquired GFP-H-Ras wt) or NK cells that were co-cultured with B721-GFP-H-Ras wt
Temperature-preconditioned myocytes were found to have significantly greater levels of pERK compared with untreated control myocytes.
Moreover, with 9L tetramer, a significant increase of pErk compared to control was seen in the 90% THEMIS KD cell line.
In Ad-eGFP-PHB infected cells, we also observed enhanced expression of pErk compared to parallel control or Ad-eGFP infected cells or treated with STS (Fig. 2A).
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Sure, he's the illegitimate son of Ned Stark, Lord of Winterfell, and was raised with all kinds of perks compared to most people, but his relative isolation is made very clear from the beginning of the series.
Co-expression of YC-H-RasG12V and YN-Gal-1 lead to a significant increase in pERK production compared to cells co-expressing YFP-H-RasG12V and Gal-1.
Baseline expression of EGFR, pEGFR, pAkt, and pERK were compared between the sensitive and resistant xenografts (Table 1, Fig. 3A D).
Furthermore, our data supports a relationship between pERK and the metastatic capacity of the cells, as adhered metastatic MDA-MB-435 and MDA-MB-231 cells contained elevated pERK levels compared to non-metastatic MCF7 and Hek-293 cells.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com