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We calculated the mean eccentricity of the peripheral dot and plotted it as a fraction of sample separation.
Contrary to that, in the 'fixate' condition, the eccentricity of the most peripheral dot differed from the sample distance.
The experiment consisted of four practice blocks and three experimental blocks containing one of three different tasks: a standard AB task, an AB task with a concurrently moving irrelevant peripheral grey dot (grey dots task), or an AB task in which a short color change in the peripheral dot had to be detected (red dot task), as described below.
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We scanned two monkeys performing a visually guided saccade task; a passive fixation task with a very similar visual display; and a passive fixation task without peripheral dots.
In agreement, immunofluorescence analysis evidenced that N-terminal SEL1L was uniquely localized in peripheral dots and along PM profiles (Figure S3A F), which were not labeled with the ER marker calreticulin.
Notably, after 30 min of stimulation, the majority of engaged receptors were concentrated in intracellular peripheral dots in control cells, while they were still localized in spots on the plasma membrane of the triple knocked-down cells (∼50% of a total of 50 cells examined).
This scrambled the coherency of movement (at a given point, dots moved in different directions) but preserved the speed gradient (central dots still moved slower than peripheral dots).
β-COP immunofluorescence at the Golgi as well as on peripheral dots (i.e., transport carriers) was also increased and 3(c)).
For the measurement of the axis of the toric lens the pupil was dilated to enable the peripheral dots on the optic to become visible.
We noticed that peripheral dots (red color) from our distribution correspond to perimeter 2 area from Bolzer et al. distribution, whereas central dots (green color) from our distribution correspond to perimeter 1 from Bolzer et. al distribution.
The optic is marked with 3 peripheral dots that indicate the cylindrical axis of the lens and so enables its correct alignment with the steepest axis of the corneal astigmatism during surgery.
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