Also, be sure to follow the doctor's instructions about having periodic blood tests while taking arthritis medications.
Patients received verbal instructions, written reminders, and periodic phone calls to promote adherence to medication.
Sections (4 μm) were immunostained using mouse anti-BrdU antibody (GE Healthcare, Buckinghamshire, UK) or rat anti-BrdU antibody (AbD Serotec, Oxford, UK) according to the manufacturer's instructions and were counterstained with periodic acid-Schiff.
Moreover, to evaluate mucus production, some slides were also stained by periodic acid Schiff Base (PAS) following the manufacture instruction (Sigma Periodic Acid-Schiff (PAS) Kit).
Mucin was detected by staining with periodic acid-Schiff reagent (Sigma, St . Louis MO) as per manufacturer's instructions or with Alcian blue, pH 2.5 (Sigma).
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To assess glycogen storage and LDL uptake, liver organoids grown in EM or DM for 11 days were stained by Periodic acid-Schiff (PAS, Sigma) and DiI-Ac-LDL (Biomedical Technologies), respectively, following manufacturer's instructions.
Functional Hepatocyte Studies To assess glycogen storage and LDL uptake, liver organoids grown in EM or DM for 11 days were stained by Periodic acid-Schiff (PAS, Sigma) and DiI-Ac-LDL (Biomedical Technologies), respectively, following manufacturer's instructions.
To visualize glycogen deposits, tissue sections of 3 μm in thickness were stained with periodic acid of Schiff (PAS) kit (Sigma-Aldrich, St . Louis USA) according to manufacturer's instructions.
Using a published protocol for shoulder range of motion which included standardised starting positions, instructions and placement of inclinometer [ 17], During the study, there was periodic checking by external assessor for reliability.
