At the end of the 24 h-period, viable cells were counted (by trypan blue exclusion) and the same number of ZA treated and non treated viable cells (4.10) were seeded in the upper compartment of 500 μL cups in 1% FBS medium.
During this period, the viable cell count in the culture increased from 104 to 106 CFU mL−1.
It was found that, after a period where viable counts were not detected, bacterial populations recovered and were able to proliferate in most cases.
During this time period reproduction (viable offspring) and infection status were recorded.
Again, after a further period, the viable or colony count decreases (often to quite low levels if such starvation is carried out for extended periods).
To prolong the effective release period, a viable approach is to incorporate a retarding step before the diffusion of the biocide to the surface occurs.
Usually furnished with land grants, these communities survived the close of the mission period and remained viable social units.
On the contrary, GQ consumption yielded steady, but lower, qmAb values, in combination with an extended period of high viable cell densities.
The LD50 value obtained after 5 days of observation period for the viable cells of A. hydrophila (ATCC 7986) injected to Labeo rohita fingerlings was found to be 1.79 × 104 cfu/mL.
