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Following a period of attachment (4 h), osteoclasts were co-cultured for 5 days with 5 × 10 autologous activated γδ T cells or CD4+ T cells in the presence of 20 ng/ml rhM-CSF and 2 ng/ml rmRANKL.
Generally, pluripotent stem cells such as ESCs are plated and cultured on mouse embryonic fibroblast feeder cell layers, and following a brief period of attachment and expansion, the resulting outgrowth is disaggregated and re-plated onto another feeder cell layer [ 23].
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Candidates admitted to postgraduate diploma courses were generally required to undergo a period of professional attachment or internship in the field of nutrition during the course.
Similarly, we show loss of ribosomal nucleolar activity during the period of physical attachment to the condensed sex chromosomes and speculate an involvement in establishing a localised chromatin modifying environment.
Often a period of the attachment would be spent in an ITU/HDU setting allowing PRHOs to become familiar with patient treatment at this level and skills in recognising and evaluating critically ill patients.
Data was collected between June 2012 and May 2014 using key informant in-depth interviews, a desk review of documents and archival materials including media content from independence (1957) through to 2008, and participant observation during a 20 month period of practical attachment at the Policy Planning Monitoring and Evaluation (PPME) directorate of the MOH by one of the authors (AK).
Thus, it is possible that a significant difference becomes apparent only with relatively long periods of romantic attachment.
Multiple ATP hydrolysis cycles are likely to occur during these extended periods of microtubule attachment (see Figure 3B and data below).
SS attached in significantly lower levels than RS after a period of 20 min attachment, but only on farm eggs.
Depression during the critical period of mother-infant attachment may also adversely affect child neurodevelopment.
However, it may be possible to match perception of safety with actual clinical performance over the period of doctor's attachment.
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