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The culture supernatants were separated at the end of the incubation period from each organism and used as crude enzymes source for hydrolysis.
For the ETP analysis, we first filtered out the Milankovitch periods eccentricity (100-ka period), tilt or obliquity (41-ka period), and precession (23-ka period)—from each of the proxies, respectively.
In order to quantify differences in development time between dl and wt, eggs were collected during a four-hour period from each of the adult colony cages and reared to adulthood.
At 48 hr after ischemia induction, (at the end of reperfusion period), from each mouse about 1.5 ml of blood was collected from the heart.
These parameters were compared in a 3-year period from each before and after the introduction of the 2005 CPR guidelines.
The middle 5 s of the 6-s delay period from each trial were submitted to multitaper spectral analysis, to estimate the power spectrum.
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Then, we subtracted the recorded angle during previous periods from each individual's pitch angle data over the period of logger deployment.
Period-10 (10.00–10.33) occurrences in the motifs were counted in a sliding window that extended ± 51 nucleotides (5 periods) from each VWG/CWB position.
The collection periods from each laboratory were staggered throughout the year, to balance workload in the HPV Reference laboratory at the Royal Infirmary of Edinburgh.
20 consecutive measurements were taken, each after 60 min equilibration periods, from each of the tonometers at PCO2's of 2.5, 5. 7.5, 10 kPa and processed in the IL BGE blood gas analyser.
It was retrospective, but the risk of this influencing results was reduced by blinding the reviewers to the period from which each letter had been sampled.
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