The critic Maxwell Geismar was not overstating when he credited Lardner with being "one of the most savage and merciless satirists and social critics of his period who dissected Babbitt before Babbitt was born".
At the end of each infection period, tongues were dissected, formalin-fixed and embedded in paraffin.
Following this period, tissue was dissected, fixed in 4% PFA in PBS-Tx 0.3%) PBS-Tx 0.3d permeabilized in 2 N HCl, PBS-Tx(0.3%) at 4°C for 30 min. Tissue wandblocked (1% Bovine Serum Albumin (BSA), 1% lysine, 1% glycine in permeabilized 4°C, 1 hr), incubated in anti-BrdU monoclonal antibody (supplied with labeling kit, 1∶10 dilution in blocking solution; 4°C, overnight).
After the experimental period, hearts were dissected out and perfused in chilled PBS.
In this period, the SACP and the adjacent continental shelf that were exposed during regression periods were deeply dissected by paleochannels that carried water from inland basins to the Atlantic ocean, before the formation of the Patos and Mirim Lagoons.
After the incubation period, neonatal brains were dissected immediately or after a period of recovery (6 or 18 h).
L4 larvae (46 48 hr post-L1) were fed on 5-ethylnyl-2′-deoxyuridine (EdU -labeled bactEdU -labeled indicated time periods and then dissected to extrude the germline for fixation.
Optical sections were chosen for each vLN based on Anti-PERIOD ICC in brains dissected between zeitgeber time 22 and 24, times when PERIOD expression is predominantly nuclear in the vLNs [33].
After the end of the last acquisition period the brains were dissected from the skulls and the activity determined using the activimeter.
At the end of each study period, the aortas were dissected and aortic RNA was isolated and used for microarray analysis to compare the acute and chronic responses of the pro-atherogenic stimuli on transcriptional profiles (For experimental protocol, see Additional file 11: Figure S7).
