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Afterwards, they were transcardially perfused with phosphate buffered saline followed by perfusion with 4% paraformaldehyde.
Results: Magnetic resonance images showed that perfusion with retrograde cardioplegic solution was heterogeneous.
Fig. 1 Dynamic SPECT imaging of pulmonary perfusion with 99mTc-PulmoBind in human.
During the perfusion with the preservation solution, respiratory rate was decreased and FiO2 increased to 100%.
Hypoxic pulmonary vasoconstriction (HPV) matches lung perfusion with ventilation which tends to optimize pulmonary gas exchange.
(B) Perfusion with 4% paraformaldehyde (PFA) for 20 min and postfixation in 4% PFA (2 h).
(C) Perfusion with 4% PFA for ~5 min, postfixation in Zamboni (2 h).
Brains were harvested after perfusion with PBS.
After 24 hours, lungs were isolated after perfusion with PBS.
and euthanized by transcardial perfusion with 4% paraformaldehyde.
Pathology analyses were performed after intracardial perfusion with 4% PFA.
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