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Microfluidic perfusion systems are used for assessing cell and tissue function while assuring cellular viability.
The ex vivo organ perfusion systems provide unique opportunities to test the effectiveness of therapeutics on human organs before being used in patients.
Perfusion systems generate more physiologically relevant and sustainable adipose tissue models, however adipocytes have unique properties that make culturing them in a perfusion environment challenging.
Microfluidic perfusion systems enable small-volume cell cultures under precisely controlled microenvironments, and are typically developed for cell-based high-throughput screening.
To do so it would require the combined mastery of chemical gradients, matrix properties, cell patterning, microfabrication, perfusion systems, and coculture techniques.
Spinner flask, rotating wall bioreactors, and perfusion systems have all been the focus of experiments, and each system has advantages and disadvantages.
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The oxygenation chamber in our perfusion system maintains perfusate Po2 > 600 mmHg.
The heart was trimmed of noncardiac tissue and retrograde perfused via a noncirculating perfusion system at a constant flow rate.
Medium can be perfused through these three devices using a gas driven perfusion system as previously required.
The cells were continuously perfused with the HBSS solution and chemicals were added via a perfusion system.
The pulsatile perfusion system enhanced the SMCs and ECs proliferation.
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