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Because perfusion speed was unknown in 74% of the cohort, we were not able to analyze the link between perfusion speed and natremia correction speed.
We could not determine whether insufficient correction of hypernatremia was due to a low speed of perfusion because perfusion speed was missing in 74% of the cohort.
In 74% of the medical files perfusion speed was missing; in 6% the perfusion setting by the nurse was not reported.
Recordings were performed at 34 37°C using an inline heating system (Warner Instruments, Hamden, CT, United States) with perfusion speed maintained (4 6 ml min−1).
Besides the too slow perfusion speed, mistakes in the type of solute chosen for rehydration could at least partly explain under-correction, as 22% of patients with MBP ≥70 mmHg either received isotonic solute or were not perfused.
Currents were normalized to the current observed in solution at a flow rate of 6 mL/min, which was subsequently set to 80% saturation as measured with the optode at this perfusion speed.
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Perfusion speeds were 6, 3 or 1.8 mL/min.
After recording a baseline of at least 15 seconds, the perfusion pump, with speed pre-set, was turned on.
The animals were deeply anesthetized with pentobarbitone (0.05 mg g−1) and perfused via the heart, using a perfusion pump, at a speed of 7 ml/min with 2.5% glutaradehyde, and 2% paraformaldehyde, in phosphate buffer (0.1 M, pH 7.4, 250 300 ml per animal).
GE light speed 16-perfusion scan was performed for each rabbit.
The speed of the perfusion was controlled by using a peristaltic pump (Instech Laboratories Inc ,Plymouth Meeting, PA).
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