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An intravenous (IV) perfusion line was used for hydration and injection of the radiotracer.
This comprised three orthogonally arranged lead collimated Na/I γ-radiation detectors (Raytest Isotopenmessgeräte GmbH, Straubenhardt, Germany) measuring 64Cu activity at the input (arterial) perfusion line, the heart and the output perfusion line.
A second long 19G/88mm-needle 19G/88mm-needle into the vial septum until touching its bottom -was connectedeeplyhe venous perfusinserted gointothethe patient.
Buffer oxygen saturation was monitored throughout each experiment by an OxyLite™ fluorescent oxygen probe (Oxford Optronix Ltd., Oxfordshire, UK) inserted into the arterial perfusion line.
Automated "quick change" might be easily improved by integrating the dead space of the perfusion line into the smart pumps' algorithms.
After a stabilisation period, where the KHB perfusate was gassed with 95%O2-5O2-5%2, the first bolus of either [18 F]-FDG or [18 F]-FMISO was injected into the perfusion line.
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In our study, the perfusion lines were set up in a standardized manner and in the same way in all study patients.
After the first stimulation (S1), the study drug capadenoson at concentrations of 30 µg/l (6 · 10−8 M) or 300 µg/l (6 · 10−7 M), or 2-chloro-N6-cyclopentyladenosine (CCPA, 10−6 M, Sigma-Aldrich), respectively, were added via separate perfusion lines for 30 minutes.
Water jacketing of perfusion lines ensured a constant temperature of 37°C and minimized the formation of air bubbles, which pose a threat to the vessel segment.
Fill the perfusion system with the buffer 1 and buffer 2. Make sure that the perfusion lines are filled and the temperature is calibrated to keep them at 37 °C.
Vascular endothelial cells seeded in the lumen of the channels by perfusion can line the channel wall and migrate into the non-sacrificial hydrogel after 3 days.
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