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The catheter was perfused with CNS perfusion fluid at 0.3 μl min−1 using a CMA106 pump.
At expected time points after ischemia, rats were deeply anesthetized with 10% chloral hydrate and transcardially perfused with heparinized saline until colorless perfusion fluid was obtained from the right atrium.
Prior to the therapy experiment with HIPEC, we investigated the intraperitoneal distribution of the perfusion fluid using a methylene blue stained perfusate.
In short, the microdialysis probes in striatum, lateral ventricle, and cisterna magna were perfused with different concentrations of acetaminophen (50, 200, and 1,000 ng/ml) in perfusion fluid.
After a first period of stabilization and DA baseline recording, KCl (75 mM) was added to the perfusion fluid of both capillaries.
Results: To address loss of perfusion fluid 4% of dextran was added and high and constant amounts of dialysate were achieved.
A collecting cannula was inserted into the lumen of the jejunum 10 cm further down to collect the perfusion fluid.
After a seal of 2 GΩ was obtained, the perfusion fluid was changed to solution B before current recording.
The perfusion fluid contained ≤0.1% DMSO to help solubilize the indole diterpenoid compounds, as used in previous in vitro experiments [30], [9], [31].
Microdialysis perfusion fluid was prepared as described above.
Isotonic perfusion fluid (Perfusion Fluid CNS; M-Dialysis, Stockholm, Sweden) was pumped through the system at a flow rate of 0.3 μL/minute.
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