To investigate whether norepinephrine can be degraded directly by the kidney, and whether perfusion flow or pressure can influence its clearance rate, we constructed isolated perfused rat kidney model as previously described [9], [10].
Yue, S., Lee, P. D., Poologasundarampillai, G. & Jones, J. R. Evaluation of 3-D bioactive glass scaffolds dissolution in a perfusion flow system with X-ray microtomography.
We addressed engineering of TLS by culturing cancer cells in porous scaffolds under perfusion flow.
The bioreactor design and perfusion flow rate are optimized by computational fluid dynamics simulation and experimentally.
Three alternative culturing schemes that convect media were considered: a spinner flask, a rotary vessel, and a perfusion flow system.
After 1 h of recovery in the measurement buffer (aCSF; CO2, 5%; O2, 95%; pH, 7.4; flow rate, 2 ml min−1), stimuli were administered into the perfusion flow via a dedicated perfusion system (TSE-systems).
Recovery values between 4 and 8% were obtained using a perfusion flow rate of 2 μL/min.
With the optimal perfusion flow rate, shear stress was minimized to achieve high and uniform cell viabilities across different plates in the bioreactor.
Our findings advocate the importance of perfusion flow in 3D cultures of tumor cells to efficiently mimic functional features observed "in vivo" and to test anticancer compounds.
It may allow specific fluid velocities and shear stresses to be determined to optimize the perfusion flow rate, porous scaffold architecture and distribution of in vitro tissue growth.
Despite wide variations in perfusion flow and systemic arterial pressure, CBF is unchanged.
