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Additionally, bioreactors that apply compression or rotation during the cartilage engineering process and different perfusion devices have been demonstrated.
Pulsatile tube adaptable to conventional perfusion devices could induce homogenous, downstream, and nearly physiologic pulsatile perfusion flow, with lower energy losses.
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Solutions were perfused directly over the cells using a gravity fed eight way perfusion device (Warner Instruments, Hamden, CT) with electrically controlled solenoids (The Lee Company, Westbrook, CT).
Reagents were applied with an automated perfusion device.
Glutamate was applied with a piezoelectric-controlled perfusion device to neurons voltage-clamped at −70 mV.
We used a perfusion device for the renewal or exchange of the culture media, consisting of 2 Teflon tubes (Tygon, Fisher Bioblock SA) and of a peristaltic pump (Ismatec).
The tissue perfusion device, with a microdelivery capillary, was fabricated following standard photolithography procedures.
The butterfly needle of the perfusion device was inserted into the left ventricle, and the right atrium was punctured.
The perfusion device could be reused by cleaning thoroughly with DI water and isopropanol after each experiment.
2ml of standard phosphate buffer solution (PBS) was injected from the perfusion device at the rate of 1-2ml/min for 5min.
After dissection from surrounding tissues, 2.5 to 3.0 cm long segments of rat tail arteries were cannulated and connected to a perfusion device.
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