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Solutions were perfused directly over the cells using a gravity fed eight way perfusion device (Warner Instruments, Hamden, CT) with electrically controlled solenoids (The Lee Company, Westbrook, CT).
Reagents were applied with an automated perfusion device.
Glutamate was applied with a piezoelectric-controlled perfusion device to neurons voltage-clamped at −70 mV.
We used a perfusion device for the renewal or exchange of the culture media, consisting of 2 Teflon tubes (Tygon, Fisher Bioblock SA) and of a peristaltic pump (Ismatec).
The tissue perfusion device, with a microdelivery capillary, was fabricated following standard photolithography procedures.
The butterfly needle of the perfusion device was inserted into the left ventricle, and the right atrium was punctured.
Similar(42)
Additionally, bioreactors that apply compression or rotation during the cartilage engineering process and different perfusion devices have been demonstrated.
Pulsatile tube adaptable to conventional perfusion devices could induce homogenous, downstream, and nearly physiologic pulsatile perfusion flow, with lower energy losses.
By applying this approach to thin gold rods aligned in a spatially controlled manner in a perfusion culture device, human umbilical vein endothelial cells (HUVECs) were transferred onto the internal surface of capillary structures in collagen gel.
The objective of this study was to establish the quantitative relationships between the cell seeding efficiency and the initial vacuum pressure in a compact perfusion seeding device that uses the effect of differential pressure induced by vacuum to seed cells on a porous scaffold.
Aastrom Biosciences developed an automated continuous perfusion culture device for expansion of hematopoietic stem cells (HSCs).
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