The lung circulation was perfused with a physiological salt solution (PSS) containing albumin (4 g/100 ml perfusate) via the pulmonary artery, using a peristaltic pump.
Tumours were perfused with a physiological buffer of known viscosity and changes in perfusion pressure were recorded at different perfusion rates in an ex vivo system.
Additionally, 4 week animals were perfused with a radiopaque material to visualize and quantify blood vessel formation.
They will have their heads frozen when they are declared dead, perfused with a cocktail of "antifreeze" chemicals, and preserved in liquid nitrogen at -196C.
After recording baseline calcium, fibers were perfused with a modified PSS+ containing high concentration of KCl (125 mM) and reduced concentration of NaCl (10 mM).
After a survival time of 7 8 days, the animals were perfused with a fixative and brain tissue was prepared for histological analysis.
Animals were sacrificed at 7, 21, 35, and 49 days (0, 4.7, 7, and 7 mm of distraction) at which time lower extremity vessels were perfused with a 60% (w/v) barium sulfate suspension.
Then, they were tracheotomized, ventilated and perfused with a central vein catheter inserted via the left femoral vein.
Corneas were perfused with a control solution (BSS Plus for humans, glutathione bicarbonate Ringer's [GBR] for rabbits) for a 1-hour stabilization period.
In the study, rat eyes were enucleated immediately after death and perfused with a tracer (Evans blue) for 40 min. Then, the anterior segment was dissected and flat-mounted on a Petri dish with TM facing upwards.
The second test group was perfused with a solution of 4% PFA in 10 mM (PBS, pH 7.4) for 20 minutes, postfixed in the same fixation solution for two hours and stored in 10 mM PBS for 24 hours.
