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CDL determined concentrations of sevoflurane in the perfusate using gas chromatography-mass spectrometric analyses.
Net water absorption, Jv (nl min-1 mm tubule length-1), was determined in each collection period with [C]dextran in the perfusate using the following relationship: Jv= (Vi - Vo)/L.
ASA and SA concentrations were measured in the microdialysis perfusate using a validated HPLC method with limits of detection at 53 ng/mL and 84 ng/mL and limits of quantification at 106 ng/mL and 169 ng/mL for ASA and SA, respectively.
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A possible alternative to intermittent sampling of the bath perfusate uses calcium sensitive electrodes to monitor bath concentrations continuously but relies on a indirect measure [15].
In comparison, Stofan et al. (52) injected rats with oxypurinol (50 mg/kg ip) 12 h before the study, and an additional dose of oxypurinol (50 mg/l) was added to the diaphragmatic perfusate used during the in vitro experiments.
Contrary to expectations, however, neither inclusion of survival factors in the final re-equilibration perfusate used immediately prior to aTP or continuation of aspirin treatment alone for 5 days after aTP showed any evidence of having a beneficial effect on the rate of restoration of fertility following WOCP&TP.
Levels of 6-keto prostaglandin (PG) F1αand thromboxane (TX) B2 were determined from mesenteric perfusates using enzyme immunoassay kits.
Therefore the reason for the observation that our ERG gives slightly smaller τD values than suction pipette recordings may lie in the minor differences in the perfusates used in these experiments, especially in the differences in pH buffers.
In these instances [3H] and [14C] radioactivity was determined in the same perfusate samples using a Tri-Carb 1900TR Liquid Scintillation Analyzer and double-label DPM software.
Allatostatin (1 µM) was added to the perfusate for 10 15 min. Fresh perfusate was used to washout the peptide.
At doses of 5 to 10 nM, ET-1 caused pulmonary edema in isolated rat lungs perfused with salt solution while no change was observed when a blood perfusate was used [ 32- 34].
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