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Single-cell qRT-PCR was performed as detailed below following a published study19 with modifications.
For that, a series of tests was performed as detailed in UCLA [30].
Oligonucleotide aCGH processing was performed as detailed in the manufacturer's protocol (CGH-v4_91; http://www.agilent.com).
Fluorescence microscopy was performed as detailed by Alayari et al. [9].
Oligonucleotide aCGH processing was performed as detailed in the manufacturer's protocol (version 4.0; http://www.agilent.com).
Normalization and statistical analysis of microarray data were performed as detailed below.
Expression profiling was performed as detailed in the supporting information (Method S1).
Proton NMR spectroscopy was performed as detailed in a previous report [22].
FA was performed as detailed above, 1 and 2 weeks after CNV creation in all treated and control animals.
In an attempt to detect proteins that interact with KCNQ1 we applied protein-protein interaction analyses, performed as detailed in [28].
To characterize the M6 allelic series, PCR reactions on genomic DNA from homozygous flies were performed as detailed in Table S1.
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