Exact(8)
After the samples arrive at the laboratory, additional operations might be required prior to performing the assay.
The reliability of these results was confirmed by performing the assay with known DnaK substrate peptides (PhoA2 21 and NR peptide 47 (Fig. 7d).
Sometimes it is necessary to change the concentration of the analyte prior to performing the assay so that it will fall within the range of the analytical method.
Spatial multiplexing – detection of two analytes in different regions of the planar surface – was also demonstrated using fluid handling constructs that allowed for very low solution volumes when performing the assay.
One day before performing the assay, liver spheroids and monolayer cultures were washed three times with phosphate buffered saline (PBS; Corning) and cultured in assay medium, consisting of Williams E (PAN-Biotech) with 5.5 mM glucose and 0.1% bovine serum albumin (Serva, Heidelberg, Germany), overnight.
The pH optimum of the endoglucanase activity of each fungus was determined by performing the assay at different pH values (Figure 2).
One concern about phenotypic assays, such as the mutagen sensitivity assay, has been that there could be wide variation in results depending on the timing of the assay (within individual variation), the individual performing the assay (within observer variation) and the laboratory where the assay has been performed (inter-laboratory variation).
Effect of pH on the activity of the pure enzyme was determined by performing the assay in a pH range from 5 to 9. The enzyme was optimally active at pH 7.5 and exhibited more than 50% of its optimal activity between pH 6.5 and 9.0 (Figure 3a).
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