Sentence examples for performing restriction from inspiring English sources

Standard methods for performing restriction digests, ligation reactions and plasmid isolations were used.

These colonies were then used to isolate plasmid and to analyze junction sites by performing restriction profile and PCR amplification.

Plasmids were isolated from the resultant colonies, and the formation of a cointegrate due to site-specific recombination between attP and attB was determined by performing restriction analysis.

U93717.1), the ADE2 marker in pRS402 is flanked by BglII sites and is thus easily replaced; however, we discovered disagreements between the actual and the GenBank sequences of pRS402 when performing restriction analysis and Sanger sequencing.

Performing restriction landmark genomic scanning (RLGS) they identified 34 differentially methylated CpG islands, which revealed neural specific CpG island hypermethylation compared with mesoderm- or endoderm-derived tissues.

Products were purified through gel extraction, and the recombinant plasmids were transferred into Escherichia coli DH5α and then identified by performing restriction-enzyme digestion and sequencing analysis.

We isolated plasmids from randomly picked colonies and performed restriction mapping.

4. Design and perform restriction digests to identify plasmids containing the expected insert.

The Los Angeles County Public Health Laboratory performed restriction fragment length polymorphism (RFLP) analyses on the isolates.

To determine whether the amplified fragments corresponded to AML cDNAs, we performed restriction digests on the PCR products, which confirmed that the cDNAs originated from AML gene transcripts.

To examine the efficiency of target mutations in the injected embryos, we performed restriction enzyme digestion of PCR products using HinfI (TaKaRa) or Hpy188I (NEB).