Exact(2)
She speaks with a woman who, in keeping with an ancient tradition, is hired to offer lamentations at funerals, performing loss for those who are unable to do so, mired as they are in the stillness of grief.
The efficiency rates we have obtained are sufficiently high that one could easily contemplate using TALEN technology as a low cost alternative to antisense morpholino oligonucleotides (MOs) for performing loss of function experiments in Xenopus.
Similar(58)
Here, we provide insights into experimental design and procedures permitting successful application of this technique to Xenopus researchers, and offer a general strategy for performing loss-of-function assays in F0 and subsequently F1 embryos.
We therefore assessed PRDM5 function in vivo by performing loss-of-function and gain-of-function experiments in zebrafish embryos.
Future studies must focus on verifying and elucidating the biological function of these CaRGA genes using supplementary experimental approaches, particularly with virus- or Agrobacterium-mediated transient assays [ 57] or by performing loss-of-function experiments, such as virus-induced gene silencing [ 58].
Split-connection protocols break TCP connection into two parts, at the base station; link-layer variants provide reliability through local retransmissions; finally, in end-to-end approaches, the sender performs loss recovery without breaking the semantics of TCP.
To gain a mechanistic understanding of their function, we mapped OSKM-binding, stage-specific transcription factors (TFs), and chromatin states in discrete reprogramming stages and performed loss- and gain-of-function experiments.
To that end, we have examined its spatiotemporal expression during vertebrate cardiac development and performed loss of function experiments.
In addition, we performed loss of heterozygosity (LOH) analysis of the APC and MCC genes.
Next we performed loss of function analysis of αv integrin in basal-like breast cancer lines.
To evaluate the loss of C3CER1 in human tumor tissues we performed loss of heterozygosity (LOH) analysis of 159 breast tumors.
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