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This could be mediated by always performing gapped local alignment similar to that implemented within BFAST.
It is interesting to note that MAQ typically finds indels by performing gapped local alignment on paired end data.
In the reported case, the authors performed gap arthroplasty and intraoral distraction as a one-stage treatment, followed by mandibular contouring surgery and prosthetic treatment for unilateral TMJ ankylosis with facial asymmetry.
Pol λ can also perform gap filling synthesis in reconstituted NHEJ reactions in vitro.
Following the ALLPATHS-LG assembly, we performed gap closure using GapCloser [ 11] at K-mer setting of 31.
Pol λ has also been shown to perform gap filling opposite thymine glycol and a benzo[ a]pyrene-derived DNA adduct.
The BRCT domain is not critical for polymerase catalytic activity, as indicated by the ability of a pol λ variant lacking the BRCT domain to perform gap filling synthesis in vitro.
We were able to assemble the genomic reads for F. noatunensis subsp. orientalis Toba04 (Genbank accession number NC_017909) into one contig with a size of 1.84 Mbp (this included some targeted sequencing to perform gap closure; see Methods for details).
This analysis could be only conducted with BLAT, SSAHA2 and CLC (Figure 5), because the other programs cannot perform gapped alignments.
Most other methods do not perform gapped local alignment (bowtie, and SOAP), or only in exceptional circumstances with paired end data (MAQ).
DHJ performed gap-closing Sanger sequencing and executed genome quality control analysis in collaboration with ACCW.
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