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The motor capability of Nes-R26 Met -SOD versus SOD mice was further evaluated by performing footprint studies (Supplementary Figure 4).
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Oxidation of non-solvent-accessible residues can hamper data interpretation and must be considered when performing footprinting experiments.
To assess the locomotion of transgenic mice, we performed footprint analysis using an adapted open-top runway system.
We also performed footprinting analysis with RNase T1, which cleaves 3' of guanosines.
In order to help define the interaction between the 3' splice-site region and PUF60 and the U2AF65/35 heterodimer and whether these interactions change when the proteins are incubated together, we performed footprinting experiments with the AdML RNA substrate and purified proteins (Figure 5).
Thus, we sought to identify TFBS motifs in CNEs by performing phylogenetic footprinting, using the lamprey elements as a guide.
We present a novel approach, implemented in the software package BigFoot, for performing phylogenetic footprinting on greater numbers of sequences.
Scoring was based on the duration of clasping as follows: clasping >10 s = score 3, 5 10 s = score 2, 1 5 s = score 1, no clasping = score 0. Gait analysis was performed by footprint analysis as described previously [14].
To address this issue, we performed engineered footprinting and single-turnover experiments to determine where and how SRPK1 initiates phosphorylation within the RS domain.
To obtain detailed information regarding the interaction of EIAV Rev with ERRE sequences and structural motifs, we performed RNA "footprinting" experiments, using chemical probing and primer extension analysis [43], [44].
Cheng Tao Lin performed the footprinting assay.
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