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In addition, the NASBA-ELISA assay had the best performance in negative predictive value, and Youden index, while RT-PCR was the best performing assay for positive likelihood ratio and positive predictive value.
Current technology relies mostly on performing assay reactions in multiwell plates (MWP) with robotic manipulation of fluids followed by interrogation using optical plate-readers.
6 Several studies have validated the Wantai IgG assay against known positive and negative controls, compared with other commercially available assays and have found the Wantai assay to be the better performing assay, with a greater degree of sensitivity.
HumM2, the second best performing assay in the India validation was used on 10% of samples positive for total Bacteroidales and negative for BacHum, but did not yield any additional human detection, which would indicate low host source sensitivity in the study context.
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LIB participated in performing assays and in revising the manuscript.
SLN performed assays and assembled the data.
We have also performed assays on extracts from transitional tissue.
Donovan performed assays of IGF-1 and Hauschka measured osteocalcin.
ZS and JJ performed assays using the scrambled siRNA controls.
JO organized and performed assays and coordindated study participants.
An alternative approach is to perform assays of HR function.
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